NU Core ID: Bio-Layer Interferometer (BLITZ) Evanston Location: Tarry Building, Rm 7-710, Chicago Campus - Not Available Location: Cook Hall, Rm 4106, Evanston Campus Note that our facility has select sensor types (please check with us to confirm the specifics) available for purchase to users. This wavelength shift is a direct measure of the change in optical thickness (nm) of the biological layer.įor a list of all available biosensor types, please see here. Any change in the number of molecules bound to the biosensor causes a shift in the interference pattern that is measured in real time.

This interference is captured by a spectrometer as a unique spectral signature and is reported in relative intensity units (nm). Some wavelengths show constructive interference (blue), others show destructive interference (red). Reflected wavelengths are affected by the thickness of the coating on the optical layer. BLItz emits white light down the biosensor, and then collects any light reflected back. The BLI (bio-layer interferometry) technology used by BLItz provides real-time data on protein interactions. Only molecules that bind directly to the biosensor surface are detected, providing exceptional specificity for individual applications, even in crude media. These direct binding assays take place on a disposable biosensor made from a biocompatible matrix that is uniform, non-denaturing and minimizes non-specific binding. The BLItz is a micro volume instrument for characterizing the kinetics of macromolecular interactions using bio-layer interferometry with low cost disposable sensor probes.īLItz™ uses ForteBio’s Dip and Read™ label-free assays. Note: The Keck Facility does not supply HPLC columns. Quaternary pump system using a high-speed proportioning valve. Peltier-thermostated column compartment (4-80 degrees C). Programmable automatic injector for 20- 900μl sample. Peltier-thermostated auto-sampler (4-40 degrees C). This Agilent 1100 series high pressure liquid chromatography system is equipped for high sensitivity sample analysis.ĭiode-array absorbance detector from 190 to 950nm.

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